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KMID : 0363020050350020511
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 2 p.511 ~ p.524
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Osteogenic activity of an adenovirus expressing BMP-2 on Human Periodontal Ligament cells
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김경화
박윤정/이상철/김태일/설양조/이용무/구영/한수부/정종평/류인철
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Abstract
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The regeneration of lost periodontal tissue is a major goal of therapy. Periodontal ligament
cell(PDL) is a specialized connective tissue that connects cementum and alveolar bone to
maintain and support teeth in situ and preserve tissue homoeostasis. Bone morphogenetic proteins(BMPs) have shown much potential in the reconstruction of the periodontum by stimulate
new bone and new cementum formation. Limitiations of BMP administration to periodontal lesions is high dose delivery, BMP transient biological activity, and low bioavailability of factors
at the wound site. Gene delivery method can be alternative treatment strategy to deliver
BMPs to periodontal tissue. The purpose of this study is to investigate efficiency of BMP-2
gene delivery with cell-based therapy using PDL cells.
PDL cell were transduced with adenoviruses encoding either BMP-2 or Lac-Z gene. To evaluate osteogenic activity of expressed BMP-2 on PDL cells, we investigated secreted BMP-2,
cellular activity, ALPase, produced mineralized nodules. To evaluate collagen scaffold as carrier for transduced cell delivery, we examined morphology and secreted BMP-2 of transducd
PDL cells on it.
BMP-2 transducd PDL cells produced higher levels of BMP-2, ALPase, mineralized nodules
than non transduced cells. Cellular activity of transduced cells was showed similar activity to
non transduced cells. Transduce cells attached on collagen scaffold secreted BMP-2 at 7day
and was showed similar morphology to non transduced cells.
These results demonstrated that transduced PDL cells produced biologically active BMP-2
and collagen scaffold could be carrier of transducd cells.
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KEYWORD
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BMP-2, Adenovirus, PDL cell, gene delivery, collagen scaffold
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